Helpful Hints: The Transposome Strategy for Creating Knockouts in Bacteria
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چکیده
When using EZ-TN5 Transposomes to create gene knockouts or to insert open reading frames, control elements, regulatory elements and antibiotic resistances into bacteria, there are number of considerations that must be made in order to plan for a successful transposition event in vivo. The three main considerations given below should be examined prior to performing in vivo transposition reactions using the EZ-TN5 transposomes.
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